C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01) C12N 15/70 (2006.01)
Patent
CA 2299282
The construction of multicopy expression systems for methioninase are disclosed. The higher expression systems employ a promoter operably linked to two or more copies of a tandemly repeated methioninase encoding sequence. Such multicopy expression systems were found to produce unexpectedly high levels of methioninase when expressed in an appropriate host.
Cette invention concerne la construction de systèmes d'expression multicopies pour la méthioninase. Les systèmes d'expression les plus élevés utilisent un promoteur lié de manière opérationnelle à au moins deux copies d'une séquence codant la méthioninase à répétition en tandem. On a remarqué que ces systèmes d'expression multicopies produisent des niveaux de méthioninase inhabituellement élevés lorsqu'ils sont exprimés dans un hôte approprié.
Tan Yuying
Anticancer Inc.
Borden Ladner Gervais Llp
LandOfFree
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