Methods for quantitating low level modifications of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2331742

The present invention provides methods for the detection and quantitation of any modification of interest in any nucleic acid sequence. In particular, the invention provides methods for detecting and quantitating low levels of modifications of interest in DNA sequences. The methods of the invention take advantage of combining the use of nucleic acid sequence modification-specific molecules which are specific for DNA modifications, of fluorescently labelled proteins which are specific for the nucleic acid sequence modification-specific molecules, of capillary electrophoresis and of laser-induced fluorescence. The methods of the invention are useful for identifying and detecting exposure to carcinogens, in early risk assessment for cancer, and in monitoring of cancer therapy.

L'invention concerne des procédés servant à détecter et à quantifier toute modification de toute séquence d'acides nucléiques. Elle concerne, en particulier, des procédés servant à détecter et à quantifier de bas niveaux de modifications dans des séquences d'ADN. Ces procédés sont basés sur la mise en application associée de molécules spécifiques d'une modification de séquence d'acides nucléiques et, plus particulièrement, de modifications d'ADN, de protéines marquées par fluorescence spécifiques pour les molécules spécifiques d'une modification de séquence d'acides nucléiques, de l'électrophorèse capillaire et de la fluorescence induite au laser. Ces procédés sont utiles pour identifier et détecter l'exposition à des carcinogènes, pour l'évaluation précoce des risques de cancer et le contrôle d'une thérapie anti-cancer.

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