C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) C12N 5/22 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2400265
An enzyme solution for subculturing primate embryonic stem (ES) cells and a method of culturing primate ES cells is described herein. The enzyme solution comprises trypsin, calcium chloride and a serum substitute. The culturing method comprises the step of culturing primate ES cells in a solution comprising trypsin and calcium chloride, and optionally a serum substitute. The solution and method of this invention enable one to stably maintain and propagate ES cells derived from a primate, such as monkey or human, for a long period in an undifferentiated state and with a normal karyotype.
Nakatsuji Norio
Suemori Hirofumi
Japan As Represented By The President Of Kyoto University
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Reprocell Inc.
LandOfFree
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