Enzyme solution for culturing primate embryonic stem cells...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 5/22 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2400265

An enzyme solution for subculturing primate embryonic stem (ES) cells and a method of culturing primate ES cells is described herein. The enzyme solution comprises trypsin, calcium chloride and a serum substitute. The culturing method comprises the step of culturing primate ES cells in a solution comprising trypsin and calcium chloride, and optionally a serum substitute. The solution and method of this invention enable one to stably maintain and propagate ES cells derived from a primate, such as monkey or human, for a long period in an undifferentiated state and with a normal karyotype.

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