Cell-free in vitro transcription and translation of membrane...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/02 (2006.01) C07K 1/04 (2006.01)

Patent

CA 2627580

The present invention relates to synthetic membranes having membrane proteins incorporated therein and, in particular, to the cell-free in vitro transcription and translation of membrane proteins into tethered planar lipid layers. In particular, the present invention provides a new method for the preparation of such membranes. The membranes, e.g., can be used for investigation of membrane receptor/ligand interactions.

La présente invention concerne des membranes synthétiques contenant des protéines membranaires intégrées dans celles-ci, et en particulier la transcription et la traduction in vitro acellulaires de protéines membranaires en couches lipidiques planaires attachées. L'invention concerne notamment un procédé de fabrication de telles membranes, lesdites membranes pouvant par ex. être employées pour l'étude d'interactions de récepteurs/ligands membranaires.

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