Assay method for peptide specific t-cells

Details Assay method for peptide specific t-cells Assay method for peptide specific t-cells

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/576 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2272881

A method of assaying for peptide-specific T-cells comprises adding peptide to a fluid sample of fresh peripheral blood mononuclear cells, and detecting a cytokine such as interferon-y produced by T-cells that have been pre-sensitised to the peptide. The assay method is quick and cheap and is expected to be useful for the study of various disease states including Hepatitis B, Hepatitis C, tuberculosis, malaria, HIV and influenza.

Procédé de dosage de lymphocytes T spécifiques pour un peptide consistant à ajouter un peptide à un spécimen liquide de cellules fraîches mononucléaires du sang périphérique et à détecter une cytokine, telle qu'interféron gamma , produite par des lymphocytes T ayant été soumis à une sensibilisation préalable au peptide. Ce procédé est rapide, peu coûteux et susceptible d'être utile à l'étude de différentes maladies, y compris l'hépatite B, l'hépatite C, la tuberculose, la malaria, le sida et la grippe.

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