Transgenic animals

Details Transgenic animals Transgenic animals

C - Chemistry, Metallurgy

07

K

C07K 14/02 (2006.01) A01K 67/02 (2006.01) A01K 67/027 (2006.01) A61K 39/21 (2006.01) C07K 14/00 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12N 15/867 (2006.01) C12N 15/89 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2351553

The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

La présente invention porte sur des méthodes et des compositions améliorées pour la production d'animaux transgéniques. L'invention permet d'introduire des séquences d'acide nucléique exogène en position 5 dans le génome d'oeufs non fécondés (par exemple, des ovocytes en prématuration et des ovocytes en préfécondation) par microinjection d'un rétrovirus infectieux dans l'espace perivitellin de l'oeuf. Les méthodes de la présente invention permettent une plus grande efficacité dans la production d'animaux transgéniques et une proportion réduite de génération d'animaux constituant des mosaïques par rapport à la présence du transgène.

Affiliated with

Also associated with

Rating

Transgenic animals does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic animals, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic animals will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2085113