C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 17/08 (2006.01) A61L 27/00 (2006.01) A61L 27/34 (2006.01) C12N 11/08 (2006.01)
Patent
CA 2256766
The present invention is directed to support members having surfaces that are rendered hydrophilic for use as a substrate for the immobilization of bioactive species thereon. The hydrophilic surfaces are chemically stable on the support member. The surfaces are also chemically variable to provide a variety of chemically functional groups for immobilization of bioactive species thereto. The surfaces comprise polymeric surfactants attached onto the surfaces of a support member and covalently cross-linked thereon to form a first layer. Hydrophilic polymers are then attached to the first layer on the support member to form a second layer thereon. The second layer is used to enhance the hydrophilicity of a support member as well as to provide a substrate for immobilizing bioactive species thereto. Methods for forming the first and second layers are also provided. In addition, methods for attaching bioactive substances to the hydrophilic polymers are provided.
La présente invention concerne des supports dont les surfaces ont été rendues hydrophiles pour permettre de les utiliser comme substrats d'immobilisation d'espèces bioactives. Ces surfaces hydrophiles, chimiquement stables sur le support, sont par contre également chimiquement variables de façon à laisser libres des groupes fonctionnels immobilisant des espèces bioactives sur de tels supports. En l'occurrence, ces surfaces sont constituées d'agents tensio-actifs polymères adhérant aux surfaces d'un support où ils sont réticulés par covalence pour former une première couche. Les polymères hydrophiles, qui adhèrent à la première couche recouvrant le support, forment ainsi une seconde couche venant renforcer la spécificité hydrophile d'un support, mais aussi constituer un substrat apte à immobiliser sur lui des espèces bioactives. L'invention, qui concerne également des techniques de formation de la première et de la seconde couche, concerne en outre des techniques de fixation de substances bioactives sur les polymères hydrophiles.
Gore Enterprise Holdings Inc.
Gowling Lafleur Henderson Llp
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