Gene serum opacity factor

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/57 (2006.01) C07H 21/04 (2006.01) C12N 1/21 (2006.01) C12N 9/52 (2006.01) C12N 15/70 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2170725

Methods and compositions are provided for cloning and expression of serum opacity factor of Streptococcus pyogenes genes. The portion produced by the recombinant DNA techniques described herein may be employed in qualitative and quantitative testing for high density lipoprotein, as a fibronectin binding factor and for the regulation of high density lipoprotein in a mammal. The gene may further be employed as a molecular probe for accurate identification of opacity factors from various strains of Streptococcus pyogenes.

Procédés et compositions utilisés pour cloner et exprimer le facteur d'opacité sérique de gènes de Streptococcus pyogenes. La partie produite à l'aide des techniques d'ADN de recombinaison décrites ici peut être employée pour tester qualitativement et quantitativement une lipoprotéine haute densité, pour jouer le rôle de facteur de liaison de fibronectine et pour réguler la lipoprotéine haute densité chez un mammifère. On peut également utiliser ce gène comme sonde moléculaire pour identifier avec précision les facteurs d'opacité provenant de diverses souches de Streptococcus pyogenes.

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