Visually determinable atp measuring method and reagent therefor

Details Visually determinable atp measuring method and reagent therefor Visually determinable atp measuring method and reagent therefor

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) C12Q 1/00 (2006.01) C12Q 1/04 (2006.01) C12Q 1/32 (2006.01) G01N 21/29 (2006.01) G01N 21/75 (2006.01) G01N 21/78 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2445941

It is intended to provide a reagent for simplified ATP measurement allowing visual judgment whereby judgment can be easily carried out at a high sensitivity. Namely, a reagent for ATP measurement whereby a reaction of forming glucose-6-phosphate from glucose is amplified with the use of ATP contained in a sample and acetyl phosphate contained in the reagent together with glucokinase and acetate kinase contained in the reagent, and then a visible coloration reaction is induced with the combined use of glucose-6- phosphate dehydrogenase and diaphorase, thereby allowing visual judgment.

L'invention concerne un réactif destiné à une mesure ATP simplifiée permettant une évaluation visuelle, cette évaluation étant facile à effectuer à une haute sensibilité. L'invention concerne notamment un réactif de mesure ATP, une réaction de formation de glucose-6-phosphate à partir de glucose étant amplifiée à l'aide de l'ATP contenu dans un échantillon et du phosphate d'acétyle contenu dans le réactif ainsi que de la glucokinase et la kinase d'acétate contenues dans le réactif. Ensuite, une réaction de coloration visible est induite par l'utilisation combinée de déhydrogénase de glucose-6-phosphate et de diaphorase, ce qui permet une évaluation visuelle.

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