Systems and methods for enhancing fluorescent detection of...

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G01N 21/64 (2006.01) C12Q 1/68 (2006.01) C40B 70/00 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2693055

Systems and methods for enhancing fluorescent detection of target molecules in a test sample are for use with an irradiating device. First fluorophores are provided for absorption of EMF radiation, and emission of a first signal. Second fluorophores are provided for partial absorption of the first signal, and emission of a second signal distinguishable from the first signal. The fluorophores are combined with the test sample, and secured to the target molecules and relative to one another. After the first fluorophores receive the EMF radiation from the irradiating device, the first signal is detected, together with the second spectral signal if the target molecules are present in the test sample.

L'invention porte sur des systèmes et des procédés pour améliorer une détection de fluorescence de molécules cibles dans un échantillon de test, destinés à être utilisés avec un dispositif d'irradiation. De premiers fluorophores sont disposés pour une absorption d'un rayonnement de champ électromagnétique (EMF), et une émission d'un premier signal. De seconds fluorophores sont disposés pour une absorption partielle du premier signal, et une émission d'un second signal, pouvant être distingué du premier signal. Les fluorophores sont combinés avec l'échantillon de test, et fixés aux molécules cibles et les uns par rapport aux autres. Après que les premiers fluorophores reçoivent le rayonnement EMF du dispositif d'irradiation, le premier signal est détecté, conjointement avec le second signal spectral si les molécules cibles sont présentes dans l'échantillon de test.

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