Tetanus toxin fragment c based imaging agents and methods,...

Details Tetanus toxin fragment c based imaging agents and methods,... Tetanus toxin fragment c based imaging agents and methods,...

A - Human Necessities

61

K

A61K 49/00 (2006.01) A61K 51/08 (2006.01) C07K 14/33 (2006.01) G06F 19/00 (2011.01)

Patent

CA 2656104

Methods for purifying Tetanus Toxin Fragment C comprising obtaining a supernatant comprising soluble Tetanus Toxin Fragment C and purifying Tetanus Toxin Fragment C under native conditions to obtain a substantially purified Tetanus Toxin Fragment C. Imaging agents comprising a Tetanus Toxin Fragment C and a reporter, and methods thereof. Methods comprising processing confocal microscopy datasets to provide a 360 degree average fluorescence intensity profile from the center of a spheroid towards the outer edge of the spheroid.

Des procédés de purification du fragment C de la toxine tétanique consistent à obtenir un surnageant contenant le fragment C de la toxine tétanique et à purifier le fragment C de toxine tétanique dans des conditions natives pour obtenir un fragment C de toxine tétanique sensiblement purifié. Cette invention porte également sur des agents d'imagerie contenant un fragment C de toxine tétanique et un reporter ainsi que sur des procédés correspondants. Les procédés consistent à traiter des ensembles de données de microscopie confocale pour obtenir un profil de l'intensité de fluorescence moyenne à 360 degrés depuis le centre d'un sphéroïde jusqu'au bord externe du sphéroïde.

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