Process and reagent for the determination of...

Details Process and reagent for the determination of... Process and reagent for the determination of...

C - Chemistry, Metallurgy

12

Q

150/3.2, 150/15.

C12Q 1/32 (2006.01) C12Q 1/52 (2006.01)

Patent

CA 1129314

ABSTRACT OF THE DISCLOSURE A process and reagent for the determination of glutamate-oxalacetate transaminase or of glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate with the formation of .alpha.-ketoglutarate in buffered solution, wherein .alpha.-ketoglutarate formed is reacted with .gamma.-aminobutyrate in the presence of .gamma.-aminobutyrate transaminase with the formation of succinate semialdehyde, NADP is reduced with the latter in the presence of succinate-semialdehyde dehydrogenase to give NADPH and his is either measured directly or is converted with a tetrazolium salt and an electron carrier into a formazane coloured material which is then measured the reagent comprises .gamma.-aminobutyrate transaminase, succinate- semialdehyde dehydrogellase, .gamma.-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate and optionally 2 tetrazolium salt, an electron carrier and a surface- active agent.

331592

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