Chemiluminescent aminophthalhydrazide-labeled conjugates for...

Details Chemiluminescent aminophthalhydrazide-labeled conjugates for... Chemiluminescent aminophthalhydrazide-labeled conjugates for...

C - Chemistry, Metallurgy

07

D

150/1, 260/1, 26

C07D 237/32 (2006.01) C07D 209/48 (2006.01) C07J 43/00 (2006.01) G01N 33/533 (2006.01)

Patent

CA 1146937

ABSTRACT OF THE DISCLOSURE Chemiluminescent-labeled conjugates of the formula: Chemi-R-L wherein Chemi represents a moiety which is capable of under- going a change in chemical structure with the production of light, R is a linking group, and L is a specifically bindable ligand, such as an antigenic protein or polypeptide, a hapten or an antibody, or a binding analog thereof. The chemilumin- escent moiety may be luminol, isoluminol, pyrogallol or luci- ferin. Preferred labeled conjugates are of the formula: Image wherein one of R1 and R2 is hydrogen and the other is -NR3R4; R3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R4 is Image wherein ? = 1-3 and L(CO? is the ligand or analog bound through an amide bond. Intermediates produces in the synthesis of such preferred conjugates are also disclosed.

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