Quencher conjugated antifluorescer in fluorescent immonoassay

Details Quencher conjugated antifluorescer in fluorescent immonoassay Quencher conjugated antifluorescer in fluorescent immonoassay

G - Physics

01

N

167/43, 150/9

G01N 33/543 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1084838

ABSTRACT OF THE DISCLOSURE A novel sensitive method for determing quali- tatively and quantitatively the presence of a wide variety of physiologically active organic compounds (ligand) and their receptors is provided. The method employs two novel reagents: the first reagent involves bonding a compound having structural similarity to the compound to be deter- mined (ligand analog) to a fluorescing compound: the second reagent involves bonding of a quencher to a receptor for said fluorescer. The amount of the receptor-quencher conjugate (quencher conjugated antifluorescer) which can bind to the first reagent (ligand analog-fluorescer) will be dependent upon the amount of ligand or ligand receptor (antiligand) present in the medium to be assayed.

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