C - Chemistry – Metallurgy – 09 – K
Patent
C - Chemistry, Metallurgy
09
K
31/71, 150/3.2
C09K 3/00 (2006.01) C12Q 1/42 (2006.01)
Patent
CA 1110527
ABSTRACT OF THE DISCLOSURE A stabilized liquid phosphate containing diagnostic composition and generally a phosphate containing substrate used in the determination of enzymes such as alkaline phosphates and acid phosphatase. The composition actually includes a buffer composition containing a magnesium ion for activation of the enzyme and a substrate composition, which are mixed at time of determination. The buffer composition is of the proper pH so that when the buffer composition and the substrate composition are mixed, the resultant mixture will adopt the pH of the buffer composition. The stabilizer which is employed is one that will prevent hydrolysis of the phosphate from the organic substrate. Effective stabilizers include phenol and phenolic compounds and imidazole and nitro alaphatic compounds having from one through six carbon atoms.
303298
Mcfadden Fincham
Modrovich Ivan E.
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