Method for improving contrast in surface immunological tests...

G - Physics – 01 – N

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G01N 21/01 (2006.01) G01N 33/48 (2006.01)

Patent

CA 1048409

METHOD FOR IMPROVING CONTRAST IN SURFACE IMMUNOLOGICAL TESTS WITH LARGE SIZE PROTEINS Abstract of the Disclosure An immunologically inert protein is added to a solution containing a large size immunologically reactive protein (antigen), and a substrate is then immersed in the solution to form, by adsorption, a monomolecular layer of the reactive protein molecules separated from each other and surrounded by the inert protein molecules. Subsequent immersion of the coated substrate in a solution containing a small size immunologically reactive protein (antibody) specific to the first reactive protein results in a dense bimolecular layer being formed on the substrate which produces a much greater change in contrast between the single and double layers than if the large size protein molecules were closely spaced together.

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