Reagent and method for determining total calcium in body fluids

Details Reagent and method for determining total calcium in body fluids Reagent and method for determining total calcium in body fluids

G - Physics

01

N

150/11, 150/14,

G01N 31/22 (2006.01) G01N 33/48 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1044581

Abstract ? colorimetric determination of total calcium in serum and other body fluids comprising the steps of mixing the sample of body fluid with a liquid reagent consisting of 2,7-bis-(4 chloro-2-phosponobenzeneazo)- 1,8-dihydroxynapthalene-3,6-disulfonic acid, commonly known as chlorophosphonazo III, in a buffer system at pH 5.0 to 5.5, and thereafter spectrophotometrically measuring the level of color development. Potassium hydrogen phthalate is disclosed as a component of the buffer system.

230706

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