G - Physics – 01 – N
Patent
G - Physics
01
N
150/16.1, 150/16
G01N 1/30 (2006.01) C12Q 1/04 (2006.01)
Patent
CA 1112987
STAINING AND ANALYSIS OF BACTERIA Abstract of the Disclosure A bacterial staining composition and methods of analysis of both gram-negative and gram-positive bacteria are disclosed. The composition comprises a chelating agent and a basic dye, both of which are operative at a pH above about 7Ø Bacterial staining may be effected by contacting either concentrated or fluidly suspended bacteria with the composition at a neutral or basic pH. Bacteria stained with the composition and concentrated by filtration, centrifugation or the like are readily visible and their presence in a specimen may, thus, be rapidly detected. The gradations of color of the stained, concentrated bacteria correspond to the number of bacteria and semi-quantitative analysis of the bacteria may be effected by comparison with a standard. Differentiation of gram-negative and gram-positive bacteria may be accomplished by treating the stained bacteria with an organic acid wash having a pH of about 2.5 to 2.6. Such a wash completely decolorizes only stained gram-positive bacteria. Finally, a method for determining bacterial susceptibility to antimicrobial agents is provided which comprises incubating bacteria with an anti- microbial agent, staining the bacteria and thereafter comparing the color gradation of the stained, concentrated bacteria with a control or standard.
334321
Melnick Joseph L.
Wallis Craig
Baylor College Of Medicine
Gowling Lafleur Henderson Llp
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