Method of bonding enzymes to porous inorganic carriers

Details Method of bonding enzymes to porous inorganic carriers Method of bonding enzymes to porous inorganic carriers

C - Chemistry, Metallurgy

12

P

195/123, 195/35.

C12P 21/00 (2006.01) C12N 11/14 (2006.01)

Patent

CA 1037399

METHOD OF BONDING ENZYMES TO POROUS INORGANIC CARRIERS Abstract Enzymes can be coupled che,mically to various water-insoluble inorganic carriers by reacting the carriers with 4,4'-bi(methoxybenzenediazonium chloride) to form a surface of diazo groups which are subsequently reacted with an enzyme solution to immobilize the enzymes. In preferred embodiments, the inorganic carriers are highly porous, have an average pore size of less than about 1000 .ANG., and consist of 4-200 mesh porous parti- cles of materials selected from the group consisting of glass, silica, alumina, and mixtures of silica and alumina.

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