Viral antibody assay using diluted whole human blood

Details Viral antibody assay using diluted whole human blood Viral antibody assay using diluted whole human blood

G - Physics

01

N

167/40, 167/45

G01N 33/60 (2006.01) C12Q 1/00 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1140464

Abstract of the Disclosure An immunoassay method for the detection of an antibody (Ab1) to a viral antigen (Ag) wherein a whole human blood sample diluted with an isotonic aqueous solution is incubated with a solid-phase form of Ag whereby any Ab1 present in the sample becomes bound to solid-phase Ag, the resulting solid- phase Ag-Ab1 complexes are separated from the sample, a label-incorporated form of an antibody to Ab1 (Ab2*) is contacted with the separated, solid-phase Ag-Ab1 complexes, the resulting solid-phase Ag-Ab1-Ab2* complexes are separated from excess Ab2*, and the amount of the label in the separated, solid-phase Ag-Ab1-Ab2* complexes is measured as a function of the presence of Ab1 in the sample. Preferably the whole blood sample is diluted 1:20 by volume with an isotonic aqueous buffer solution. The method is particularly useful for the detection of cytomegalovirus antibody or Rubella antibody.

359949

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