Immunoassay of antigens

Details Immunoassay of antigens Immunoassay of antigens

G - Physics

01

N

167/41

G01N 33/52 (2006.01) G01N 33/531 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/78 (2006.01)

Patent

CA 1149278

ABSTRACT "IMMUNOASSAYS OF ANTIGENS" In the immunoassay of antigens in liquids, a reaction mixture is formed containing the liquid under assay, labelled antigen, and a mixed binding reagent which contains an antigen-binding site and a label-binding site, the two sites being spaced apart in the reagent so that a single molecule of labelled antigen cannot bind to both sites. The label is one whose activity is changed upon binding to a label-binding site, and the amount of antigen in the original liquid sample is determined by measuring the activity of the label in the reaction mixture. A preferred label is a fluorophore. The mixed binding reagent preferably consists of two antibodies linked together.

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