Fluorescence spectrometry using bleaching intensity

Details Fluorescence spectrometry using bleaching intensity Fluorescence spectrometry using bleaching intensity

G - Physics

01

N

73/51.5

G01N 21/01 (2006.01)

Patent

CA 1059785

ABSTRACT OF THE DISCLOSURE An improved system is desired for examining fluorescent material. The method comprises the steps of illuminating material with radiation at a fluorescent excitation wavelength of said material and at an intensity sufficient to cause bleaching of the material; detecting over a time interval commencing with initial illumination of the material, fluorescent emission produced by the material during bleaching of the latter by the radiation; and integrating over the interval the fluorescent emission detec- ted during the bleaching of the material. Means are provided for illuminating the material to cause bleaching, for detecting fluorescent emission produced during bleaching, for measuring the decay time interval, and for integrating a signal from the detecting means over a time interval.

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