C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/2
C12Q 1/54 (2006.01) C12Q 1/28 (2006.01)
Patent
CA 1068201
Abstract of the Disclosure It is known to determine the concentration of glucose in a biological sample using a number of different spectrophotometric indicators. However the known indicators have a number of drawbacks in that sensitive spectrometers are required for the test, and usually the sample must have the protein removed from it. The present invention seeks to overcome these drawbacks by providing a process for the enzymatic spectrophotometric determination of glucose in a sample comprising the oxidation of glucose in the presence of an enzyme reagent therefore and the reaction of the resulting hydrogen peroxide with an indicator system comprising at least (a) compound selected from (i) phenol which is unsubstituted or substituted by one or more of hydrogen, halogen, NH2, SO?, C1 - C3 alkyl, cyclohexyl, methoxy, carboxylic acid, lower alkyl carboxylic ester, aldehyde,phenyl which may be unsubstituted or further substituted by one or more of hydroxy, carbonyl or carboxylic acid or a radical Image ; (ii) naphthalene which is unsubstituted or substituted by one or more of hydroxyl, methyl or SO?; or (iii) quinoline which is unsubstituted or substituted by one or more of hydroxyl; CH3; SO?; or (iv) barbituric acid which may not be substituted in its 5 position; 5 pyrazolone; diketohydrindene; or 2,4-diamino-6-hydroxypyrimidine; and (b) a compound 4-aminoantipyrine. The indicator system further includes ferrocyanide.
322060
Istituto Sieroterapico E. Vaccinogeno Toscano "sclavo" S.p.a.
Na
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