Production of superattenuated beers with immobilized enzymes

C - Chemistry – Metallurgy – 12 – C

Patent

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195/9

C12C 5/02 (2006.01)

Patent

CA 1142465

ABSTRACT A method of preparing superattenuated beer comprises treating said beer with an alpha 1,6 glycosidase which has been immobilized on a solid support. The preferred alpha 1,6 glycosidase is glycoamylase which is immobilized by first oxidizing the hexose residues of the sugar portion of the glycoproteins of the enzyme to yield a protein contain- ing reactive aldehydic functional groups. The thus obtained aldehydic glucoamylase (AGA) is reacted with a water insol- uble material bearing a functional group which will coval- ently bond with the aldehydic groups of the glucoamylase under mild reaction conditions.

363467

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