C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
150/15, 195/35.1
C07K 17/14 (2006.01) C12N 11/14 (2006.01) C12Q 1/32 (2006.01) C12Q 1/66 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1094477
ASSAYS UTILIZING LOCALIZED ELECTROMAGNETIC RADIATION SOURCES Abstract of the Disclosure Chemical species including biological materials such as enzymes, substrates, antibodies, antigens and the like, are determined in very small quantities (down to picomole ranges) through the use of electromagnetic radiation generating reactions such as those catalyzed by light emitting enzyme systems of the bacterial luciferase and fire-fly luciferase types. The sensitivity of the detection systems is increased by concentrating and localizing at least one component of the electromagnetic radiation generating reaction onto a solid support material. The component may thus be immobilized and solubilized. The localized, immobilized component is generally contacted with suitable reactants in a liquid environment in order to produce the electromagnetic signals in a localized area of the reaction environment, i.e., on the support material. The signals are thereby intensified and detected on suitable instrumentation. The electromagnetic radiation generating systems may also be coupled with other reactions that yield a necessary component of said systems, whereby chemical species associated with the coupled reactions may also be determined.
277702
Kirby Eades Gale Baker
The Regents Of The University Of California
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