Method for determining bacterial endotoxin and kit therefor

Details Method for determining bacterial endotoxin and kit therefor Method for determining bacterial endotoxin and kit therefor

C - Chemistry, Metallurgy

12

Q

150/16

C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/579 (2006.01)

Patent

CA 1201048

ABSTRACT A method for determining a bacterial endotoxin in an assay sample by an amoebocyte lysate component of horseshoe crab in the presence or absence of a substrate for endotoxin determination, characterized by using an assay sample in the liquid state obtained by treating said assay sample with an endotoxin-free acid having a pKa25°C value of not more than 3 at a pH of not more than 3; and a kit therefor consisting essentially of a receptacle containing (a) an endotoxin-free acid having a pKa25°C value of not more than 3 for treating the assay sample and a receptacle containing (b) an amoebocyte lysate component of horseshoe crab.

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