Devices and kits for immunological analysis

Details Devices and kits for immunological analysis Devices and kits for immunological analysis

G - Physics

01

N

167/44

G01N 33/543 (2006.01)

Patent

CA 1200761

New devices and kits for immunological analysis Abstract The invention relates to new devices and kits for immuno-assays, especially solid-phase immuno-assays, comprising a solid porous support, preferably in the form of a sheet, where antigens or immuno- globulins, or both of them, are bound by direct application, with no other chemical or electrochemical treatment. The use of such supports makes possible to effect an unlimited number of antibody-antigen reactions simultaneously and in one operation. The assays with these new kits are technically extremely simple in practice. The antigens or immunoglobulins on the solid support can be applied in any suitable pre-selected geometry, e.g. as an array of dots, preferably micro-dots, or lines. A preferred material for the solid support is nitrocellulose or nitrocellulose mixed with other cellulose esters. Before carrying out the immuno-assays residual adsorbing sites on the support must be saturated with whole serum of heterologous species to prevent non-specific binding. The invention is also directed in particular to devices and kits treated in this manner, and, if desired, washed and dried. They can be stored for an inde- finite time without loss of activity. In the immuno-assays to be carried out according to the present- invention the preferred detection system is the use of anti-primary species antibody coupled to peroxidase with a chromogenic substrate. The color intensity can be quantitated and calibrated with standards of known amounts of immunoglobulins bound to the same support. Densitometry permits the evaluation of the color reaction over a 1000-fold range of concentrations. The new kits can also be used with specific antibodies in a pre-determined array on the solid support for the detection of specific antigens and with complement proteins to detect antigen- antibody complexes.

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