Methods for identifying alleles associated with increased...

Details Methods for identifying alleles associated with increased... Methods for identifying alleles associated with increased...

G - Physics

01

N

167/37

G01N 33/577 (2006.01) C07K 16/28 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1314811

Abstract of the Disclosure Methods for identifying individuals at increased risk of diabetes are disclosed. The methods disclosed utilize the discovery of the DQw3.2 variant, which identifies a specific allelic polymorphism at a single gene locus. One preferred method utilizes a labeled probe to detect the DQw3.2 allele. This method involves estimating the size of the hybridizable DNA fragment generated by a specific restriction endonuclease and therefrom determining the presence of the allele. A second preferred method involves the serologic detection of the DQw3.2 allele. Within this method, immunocomplexes formed between two different MAb's and separate portions of a cell collection are detected and the presence or absence of the allele determined.

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