C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2580700
Methods of isolating nucleic acids from samples of cellular material are disclosed which use solid phase binding materials and which avoid the use of a lysis solution. The use of the solid phase binding materials unexpectedly allow the nucleic acid content of cells to be freed and captured directly and in one step. The new methods represent a significant simplification over existing methods. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.
La présente invention concerne des procédés permettant d'extraire les acides nucléiques d'échantillons de matière de cellules, utilisant des matières de liaison en phase solide, en évitant l'utilisation d'une solution lytique. Contre toute attente, l'utilisation de ces matières de liaison en phase solide permet de libérer l'acide nucléique contenu dans les cellules et de la capturer directement en une opération. Les procédés de l'invention constituent une importante simplification des procédés existants. Les matériaux en phase solide préférés convenant pour les procédés et compositions de l'invention comprennent une partie se liant à l'acide nucléique à onium quaternaire.
Gowling Lafleur Henderson Llp
Lumigen Inc.
Nexgen Diagnostics Llc
LandOfFree
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