Genes identified as required for proliferation in...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) C07K 14/245 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2360085

The sequences of nucleic acids encoding proteins required for E. coli proliferation are disclosed. The nucleic acids can be used to express proteins or portions thereof, to obtain antibodies capable of specifically binding to the expressed proteins, and to use those expressed proteins as a screen to isolate candidate molecules for rational drug discovery programs. The nucleic acids can also be used to screen for homologous genes that are required for proliferation in microorganisms other than E. coli. The nucleic acids can also be used to design expression vectors and secretion vectors. The nucleic acids of the present invention can also be used in various assay systems to screen for proliferation required genes in other organisms as well as to screen for antimicrobial agents.

L'invention concerne des séquences d'acides nucléiques codant pour des protéines qui interviennent dans la prolifération cellulaire de E. coli. Ces acides nucléiques peuvent être utilisés pour exprimer des protéines ou des parties de celles-ci, afin de produire des anticorps capables de se lier spécifiquement aux protéines exprimées, et d'utiliser lesdites protéines exprimées comme crible pour isoler des molécules candidates pour des programmes de découverte de médicaments rationnels. Lesdits acides nucléiques peuvent également être utilisés pour cribler des gènes homologues nécessaires à la prolifération cellulaire dans des micro-organisme autres que E. coli. Les acides nucléiques peuvent, en outre, être utilisés pour concevoir des vecteurs d'expression et des vecteurs de sécrétion. Ils sont également utilisés dans différents systèmes d'analyse afin de cribler des gènes de prolifération requis dans d'autres organismes et pour cribler des agents antimicrobiens.

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