Process of production of a preparation for staining nuclear...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 1/30 (2006.01)

Patent

CA 2177359

A process for the production of a preparation for specific staining of nuclear DNA is disclosed, that can be used to determine the DNA cell contents by flow cytometry. The working solution of such a preparation, containing besides the specific dye, a thermolabile enzyme and a surfactant, is dehydrated under vacuum so as to stabilize it and to modify its characteristic of access to ligand and hydration, and in this way it can be stored for a long time, preferably packed in single dose vials, and then reconstituted at the moment of use, maintaining its properties perfectly unaltered.

L'invention concerne un procédé de production d'une préparation de coloration spécifique d'ADN nucléaire, que l'on peut utiliser afin de déterminer le contenu des cellules d'ADN à l'aide d'une cytométrie de flux. On déshydrate sous vide la solution de travail d'une telle préparation afin de stabiliser celle-ci et de modifier sa caractéristique d'accès au ligand et son hydratation, cette solution contenant en outre le colorant spécifique, une enzyme thermolabile ainsi qu'un tensioactif; de cette manière, cette solution peut se conserver pendant une longue durée, de préférence en flacons à dose unique, et elle peut être reconstituée lors de son utilisation, ses propriétés étant parfaitement conservées et non altérées.

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