Preparation of pure cultures of post-mitotic human neurons

Details Preparation of pure cultures of post-mitotic human neurons Preparation of pure cultures of post-mitotic human neurons

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2120730

2120730 9308266 PCTABS00021 NTera 2/cl.D1 (NT2) cells, a human teratocarcinoma cell line, were manipulated following retinoic acid (RA) treatment to yield >95 % pure cultures of neuronal cells (NT2-N cells). This culture method is capable of yielding sufficient highly differentiated post-mitotic NT2-N cells for both biochemical and molecular biological studies. NT2 cells can be transfected efficiently and the transfected gene products can be expressed in both NT2 and NT2-N cells.

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