C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/129, 530/13,
C12N 15/24 (2006.01) A61K 38/20 (2006.01) C07K 14/54 (2006.01) C12N 1/21 (2006.01) C12N 15/66 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2004261
ABSTRACT The present invention is directed to recombinant genes and their encoded proteins which are peptides with IL-6 activity. Such proteins include the cysteine-free synthetic lL-6 active peptide and the recombinant peptide with cysteines. The invention is directed to the economical commercial production of large amounts of peptides with IL-6 activity in a form which does not require the use of harsh denaturants and which does not need to be refolded after purification. The proteins of the present invention can be used to stimulate protein production by cells, including cells of the immune system and hepatocytes. The proteins of the present invention may have antiviral activity and may prevent viral infection of cells. In a preferred embodiment, the cysteine-free synthetic IL-6 like peptide is produced by microbial cells as a soluble trihybrid fusion comprising synthetic cysteine-free IL-6 like peptide, a chemically cleavable peptide and a protein capable of expression in the host organism. After purification of the large fusion protein, the synthetic IL-6 like peptide is removed by digestion of the collagen portion of the fusion with collagenase. The synthetic cysteine free IL-6 like protein is purified by HPLC to yield a pure protein that stimulates the production of immunoglobulins by B-cells, and stimulates the production of hepatic proteins.
Fowlkes Dana M.
Tackney Charles T.
Fowlkes Dana M.
Imclone Systems Inc.
Osler Hoskin & Harcourt Llp
Tackney Charles T.
Trustees Of The University Of North Carolina (the)
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