C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 5/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/02 (2006.01)
Patent
CA 2631877
The present invention relates to a method for detecting the presence or absence of a target cell in a sample, said method comprising (a) binding cells in said sample to a particulate and mixable solid support; (b) eluting the cells from the solid support without the use of competitor molecules to disrupt the interaction between the cell and the solid support; (c) after lysis of said cells, detecting the presence or absence of nucleic acid characteristic of said target cell, wherein said solid support does not have antibodies or antibody fragments immobilised thereon. Kits for carrying out the method of the invention are also provided.
La présente invention concerne un procédé de détection de la présence ou de l'absence d'une cellule cible dans un échantillon, ledit procédé comprenant les étapes consistant à (a) liaiser des cellules présentes dans ledit échantillon à un support solide particulaire et mélangeable ; (b) éluer les cellules à partir du support solide sans utiliser de molécules concurrentes pour dissocier l'interaction entre la cellule et le support solide ; (c) après la lyse desdites cellules, détecter la présence ou l'absence d'acide nucléique caractéristique de ladite cellule cible, aucun anticorps ou fragment d'anticorps n'étant immobilisé sur ledit support solide. L'invention concerne également des kits pour mettre en AEuvre le procédé de l'invention.
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