Primate embryonic stem cells

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/0735 (2010.01)

Patent

CA 2190528

A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (-); SSEA-3 (+); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a paricularly advantageous embodiment, the cells of the preparation have normal karyotypes and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

L'invention se rapporte à une préparation purifiée de cellules souches embryonnaires de primates. Cette préparation se caractérise par les marqueurs de surface cellulaire suivants: SSEA-1 (-); SSEA-3 (+); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); et la phosphatase alcaline (+). Selon un mode de réalisation particulièrement préféré, les cellules de la préparation comportent des caryotypes normaux et continuent de proliférer dans un état indifférencié après une culture effectuée en continu sur onze mois. Les lignées cellulaires souches embryonnaires conservent, par l'intermédiaire de la culture, leur aptitude à former des trophoblastes et à les différencier dans tous les tissus dérivés des trois couches de germes embryonnaires (endoderme, mésoderme, ectoderme). L'invention se rapporte également à un procédé d'isolement d'une lignée cellulaire souche embryonnaire de primates.

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