C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01)
Patent
CA 2711895
The inventions is based on an expression enhancer sequence derived from the RNA-2 genome segment of a bipartite RNA virus, in which a target initiation site in the RNA-2 genome segment has been mutated. Deletion of appropriate start codons upstream of the main RNA2 translation initiation can greatly increase in foreign protein accumulation without the need for viral replication. Also provided are methods, vectors and systems, including the 'hyper-translatable' Cowpea Mosaic Virus ('CPMV-HT) based protein expression system.
L'invention se fonde sur une séquence d'amplificateur d'expression provenant du segment de génome ARN-2 d'un virus à ARN bipartite dans lequel un site d'initiation cible du segment de génome d'ARN-2 a été muté. Une délétion de codons d'initiation appropriés en amont de l'initiation principale de traduction d'ARN-2 peut fortement augmenter l'accumulation de protéines étrangères sans nécessiter une réplication virale. L'invention porte également sur des procédés, des vecteurs et des systèmes comprenant le système d'expression de protéines basé sur le Virus de la mosaïque du niébé = hyper-traduisible = (=CPMV-HT =).
Lomonossoff George Peter
Sainsbury Frank
Fetherstonhaugh & Co.
Plant Bioscience Limited
LandOfFree
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