C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C07K 14/16 (2006.01) C07K 14/47 (2006.01) C12N 15/09 (2006.01) C12N 15/12 (2006.01) C12N 15/33 (2006.01) C12N 15/49 (2006.01) C12N 15/64 (2006.01) C12N 15/67 (2006.01)
Patent
CA 2200342
Provided are synthetic genes encoding a protein normally expressed in mammalian cells wherein at least 10% of the non-preferred or less preferred codons in the natural gene encoding said mammalian protein have been replaced by a preferred codon encoding the same amino acid. Methods for preparing such synthetic genes, comprising identifying non-preferred and less preferred codons in the natural gene encoding said protein and replacing at least 10% of said non-preferred and less preferred codons with a preferred codon encoding the same amino acid as the replaced codon are also provided. Preferred codons may be gcc, cgc, aac, gac, tgc, cag, ggc, cac, atc, ctg, aag, ccc, ttc, agc, acc, tac, and gtg, less preferred codons may be ggg, att, ctc, tcc, and gtc, and non-preferred codons may be all codons other than the preferred codons and the less preferred codons.
L'invention concerne un gène de synthèse codant une protéine normalement exprimée dans des cellules mammaliennes, dans lesquelles au moins un codon non préféré ou moins préféré du gène naturel codant la protéine mammalienne a été remplacé par un codon préféré codant le même acide aminé.
Seed Brian
Smart & Biggar
The General Hospital Corporation
LandOfFree
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