5' ests for secreted proteins expressed in endoderm

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C07H 21/00 (2006.01) C07K 14/00 (2006.01) C07K 14/47 (2006.01) C12N 15/12 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2296398

The sequences of 5' ESTs derived from mRNAs encoding secreted proteins are disclosed. The 5' ESTs may be to obtain cDNAs and genomic DNAs corresponding to the 5' ESTs. The 5' ESTs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. Upstream regulatory sequences may also be obtained using the 5' ESTs. The 5' ESTs may also be used to design expression vectors and secretion vectors.

L'invention concerne des séquences EST 5' (marqueurs séquentiels exprimés) dérivés d'ARNm codant des protéines sécrétées. Les EST 5' peuvent être utilisés pour la production d'ADNc et d'ADN génomiques correspondant aux EST 5'. Les EST 5' peuvent également être utilisés dans le diagnostic, la médecine légale, la thérapie génique et les procédures de cartographie des gènes. Des séquences de régulation d'amont peuvent également être produites au moyen des EST 5'. Les EST 5' peuvent également être utilisés pour la mise au point de vecteurs d'expression et de vecteurs de sécrétion.

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