A composition and its use for adminstration of mitomycin c...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01) A61K 31/407 (2006.01)

Patent

CA 2524179

Methods for administering mitomycin C to a multi-drug resistant cell and for reducing the toxicity of the compound are described. In the methods, mitomycin C is provided in the form of a prodrug conjugate, where the drug is linked to a hydrophobic moiety, such as a lipid, through a cleavable dithiobenzyl linkage. The dithiobenzyl linkage is susceptible to cleavage by mild thiolysis, resulting in release of mitomycin C in its original form. The linkage is stable under non reducing conditions. The prodrug conjugate can be incorporated into liposomes for administration in vivo reducing conditions or in response to administration of an exogenous reducing agent.

L'invention concerne des méthodes utilisables pour administrer la mitomycine C à une cellule résistante aux antibiotiques et réduire la toxicité du composé. Selon les méthodes de l'invention, la mitomycine C est administrée sous la forme d'un conjugué d'un promédicament, le médicament étant lié à un fragment hydrophobe, tel qu'un lipide, par une liaison dithiobenzyle clivable. La liaison dithiobenzyle peut être clivée par thiolyse douce, ce qui se traduit par la diffusion de la mitomycine C dans sa forme originale. La liaison est stable dans des conditions de non réduction. Le conjugué du promédicament peut être incorporé dans des liposomes pour être administré dans des conditions de réduction in vivo, ou en réaction à l'administration d'un agent réducteur exogène.

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