A continuous process of dynamic high-pressure homogenization...

A - Human Necessities – 23 – J

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A23J 3/08 (2006.01) A23J 1/08 (2006.01) A23J 1/20 (2006.01) A23J 3/04 (2006.01) A23J 3/14 (2006.01) A23L 1/0562 (2006.01)

Patent

CA 2259761

The present invention relates to a continuous process for denaturation of proteins, which comprises the steps of: a) subjecting a protein solution to a high pressure homogenizer at a pressure of about 500 to about 5000 bar, at a recirculation ranging from 0 to about 50 and at a temperature ranging from about 20°C to about 80°C for a period of time from about 1 milliseconds to about 10 minutes, wherein the protein solution consisting of a protein fraction dispersed in water, buffer or salt solution at a concentration ranging from about 2% to about 35% w/w and at a pH adjusted between about 2.0 to about 12.0; b) concentrating the protein solution by i) evaporation, ii) ultrafiltration and sprayed dried or iii) ultrafiltra-tion and freeze dried.

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