C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/04 (2006.01) C08B 37/00 (2006.01)
Patent
CA 2569495
The present invention relates to a simple, direct and cost-effective process for the preparation of agarose of high gel strength and low gelling temperature from naturally occurring or cultivated Gracilaria spp. more particularly Gracilaria dura, said process comprising steps of pre-treating the dry seaweed with alkali, rinsing the pre-treated seaweed until the washing shows a pH ranging between 7 and 8, adding water, autoclaving to obtain extractive, treating the extractive with charcoal and Celite to obtain hot extractive, vacuum-filtering the hot extractive over a Celite bed, freezing the filtrate into a mass and thawing the mass, redissolving the mass in water by heating in an autoclave, repeating the freeze- thaw cycle, straining the product to remove thawed liquid and thereafter squeezing to expel residual liquid to the extent possible to obtain agarose, and an agarose thereof.
Un procédé simple direct et rentable de préparation d'agarose à force en gelée élevée et à faible température de gélatinisation à partir de Gracilaria spp. naturelle ou cultivée, plus particulièrement Gracilaria dura. Le procédé comprend les étapes de prétraitement de l'algue séchée avec un alcalin, le rinçage de l'algue prétraitée jusqu'à ce que le lavage montre un pH compris entre 7 et 8, l'ajout d'eau, la stérilisation afin d'obtenir un extrait, le traitement de l'extrait avec du charbon et Célite afin d'obtenir un extrait chaud, le filtrage sous vide de l'extrait chaud sur un lit Célite, la congélation du filtrat en une masse et le ramollissement de celle-ci, la redissolution de la masse dans l'eau par chauffage dans un autoclave, la répétition du cycle congélation-dégel, la percolation du produit afin de retirer le liquide et la compression pour extraire le liquide résiduel dans la mesure du possible afin d'obtenir une agarose et l'agarose ainsi obtenue.
Eswaran Karuppanan
Ghosh Pushpito Kumar
Mantri Vaibhav Ajit
Meena Ramavatar
Prasad Kamalesh
Council Of Scientific And Industrial Research
Gowling Lafleur Henderson Llp
LandOfFree
A cost-effective process for preparing agarose from... has received 1 rating(s) and 1 review(s), resulting in an average rating of 4.80 on a scale from 1 to 5. The overall rating for this patent is superior.
If you have personal experience with A cost-effective process for preparing agarose from..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A cost-effective process for preparing agarose from... will most certainly appreciate the feedback.
R meena
Excellent invention in this area specifically for indian resources and related industries.
Was this review helpful to you? Rating [ 4.80 ]
Profile ID: LFCA-PAI-O-1712721