A device for performing an assay, a method for manufacturing...

B - Operations – Transporting – 01 – L

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150/8, 150/8.5

B01L 3/00 (2006.01) B01J 19/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/551 (2006.01)

Patent

CA 2290945

The present invention relates to a device for performing an assay, which device comprises a substrate having oriented through-going channels, said channels opening out on a surface for sample application, the channels in at least one area of the surface for sample application being provided with a first binding substance capable of binding to an analyte. The object of the present invention is to provide a substrate having both a high channel density and a high porosity, allowing high density arrays comprising different first binding substances to be applied to the surface for sample application. More in particular, the object of the present invention is to provide a device comprising a relatively cheap substrate that does not require the use of any typical microfabrication technology and, that offers an improved control over the liquid distribution over the surface of the substrate. The above objects are achieved with a device as mentioned above wherein the porous substrate is an electrochemically manufactured metal oxide membrane.

Cette invention concerne un dispositif permettant d'effectuer un dosage, ce dispositif comprenant un substrat comportant des canaux traversants orientés. Les canaux s'ouvrent sur une surface pour effectuer l'application d'échantillon, les canaux situés dans au moins une zone de la surface pour l'application d'échantillon étant pourvus d'une première substance de liaison capable de se lier à un analyte. Cette invention a pour objet de produire un substrat présentant à la fois une forte densité de canaux et une porosité élevée pour permettre de réaliser des réseaux haute densité comprenant différentes premières substances de liaison devant être appliquées sur la surface destinée à l'application d'échantillon. Plus particulièrement l'objet de cette invention est de produire un dispositif comprenant un substrat relativement bon marché qui ne nécessite pas l'utilisation d'une technologie spécifique de microfabrication et qui offre une meilleure régulation de la distribution du liquide sur la surface du substrat. Cette invention atteint les objectifs recherchés avec un dispositif dans lequel le substrat poreux est une membrane à l'oxyde métallique fabriquée par un processus électrochimique.

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