C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C12N 1/06 (2006.01)
Patent
CA 2220867
A process is disclosed for the large scale isolation and purification of plasmid DNA from large scale microbial fermentations. All three forms of plasmid DNA; supercoil (form I), nicked or relaxed circle (form II), and linearized (form III), are individually isolatable using the disclosed process. Highly purified DNA suitable for inclusion in a pharmaceutical composition is provided by the disclosed process.
L'invention concerne un procédé d'isolation et de purification à grande échelle d'ADN plasmidique à partir de fermentations microbiennes importantes. Ce procédé permet d'isoler individuellement les trois formes d'ADN plasmidique: superhélice (forme I), cercle crénelé ou relâché (forme II) et ligne droite (forme III). Il permet également d'obtenir de l'ADN extrêmement purifié qu'on peut introduire dans une composition pharmaceutique.
Lee Ann L.
Sagar Sangeetha
Merck & Co. Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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