A method for measuring a marker indicative of the exposure...

G - Physics – 01 – N

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G01N 33/566 (2006.01) A61K 51/04 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2404540

The present invention demonstrates that epibatidine binds to nicotinic receptors on leukocytes. Epibatidine is a sensitive detector of increases in the number of nicotinic receptors expressed by a cell. Epibatidine binds to the leukocytes of smokers, but does not bind to the leukocytes of non-smokers. Both in vivo and ex vivo nicotine exposure induces epibatidine receptor expression. Epibatidine binding to leukocytes reflects epibatidine binding in the central nervous system, which in turn reflects nicotine-induced effects on the central nervous system. Epibatidine binding assays in peripheral blood leukocytes can be used to evaluate an individual's exposure to, and dependence on, nicotine.

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