A method of plant tissue culture and regeneration

Details A method of plant tissue culture and regeneration A method of plant tissue culture and regeneration

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01H 4/00 (2006.01)

Patent

CA 2170760

Plants may be regenerated from stomatal cells or protoplasts of such cells. Prior to regeneration the cells or protoplasts may be genetically transformed by the introduction of hereditary material most preferably by a DNA construct which is free of genes which specify resistance to antibiotics. The regeneration step may include callus formation on a hormone-free medium. The method is particularly suitable for sugar beet.

Des végétaux peuvent être régénérés à partir de cellules stomatiques ou de protoplastes de ces cellules. Avant leur régénération, les cellules ou protoplastes peuvent être génétiquement transformés par l'introduction d'un matériau héréditaire, idéalement par un produit de recombinaison d'ADN qui est dépourvu de gènes ayant pour spécificité de conférer une résistance aux antibiotiques. L'étape de régénération peut comporter la formation de cal sur un milieu dépourvu d'hormones. Le procédé est notamment approprié pour la betterave à sucre.

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