A method of restoring contaminated soil

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/00 (2006.01) B09C 1/10 (2006.01) C02F 3/34 (2006.01) C12N 11/00 (2006.01) C12N 11/02 (2006.01)

Patent

CA 2342805

A method for recovering soil polluted with organic compounds (pesticides, etc.) by degrading the organic compounds with a bacterium and for preventing underground water from pollution. A method for accumulating a bacterium capable of degrading specific organic compounds onto a porous material provided with a number of pores onto which the organic compounds can be adsorbed and in which the bacterium can easily grow. In this method, the porous material is inoculated with the isolated degrading bacterium alone to form a accumulation layer made of the thus inoculated porous material. Next, an inorganic salt medium containing the above-mentioned organic compounds exclusively as a carbon and nitrogen sources is refluxed through the accumulation layer to thereby accumulate the bacterium onto the porous material at an elevated rate. The carrier holding the degrading bacterium thus formed is directly embedded in the polluted soil.

Cette invention, qui a trait à une technique de réhabilitation de sol pollué par des composés organiques (pesticides, etc.), consistant à décomposer les composés organiques à l'aide de bactéries, concerne également une méthode de prévention de la pollution d'eau souterraine. Elle porte sur un procédé permettant d'accumuler des bactéries capables de décomposer des composés organiques spécifiques sur un matériau poreux dans les pores duquel les composés organiques sont adsorbés et dans lequel les bactéries se développent facilement. On introduit, dans le cadre de ce procédé, les bactéries de décomposition isolées de manière à former une couche d'accumulation constituée par le matériau poreux ayant subi cette inoculation. On injecte ensuite un milieu à base de sel inorganique contenant les composés organiques seulement comme sources de carbone et d'azote dans la couche d'accumulation afin de faire s'accumuler les bactéries sur le matériau poreux sous une concentration élevée. Le support porteur des bactéries de décomposition ainsi formé est directement enfoui dans le sol pollué.

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