A robust, inducible cardiac preferred expression system for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A01K 67/027 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2491687

The methods and compositions of the present invention find use in altering cardiac-preferred expression in transgenic animals. The compositions of the invention include isolated nucleic acid molecules, expression cassettes, animal cells, transgenic animals, and transgenic mice. The transgenic animals of the invention exhibit inducible cardiac preferred expression of a nucleotide sequence of interest. The methods allow generation of transgenic animals with altered cardiac preferred expression of the nucleotide sequence of interest. In particular, the invention provides a method for altering the susceptibility of a transgenic animal to cardiopathy. A transgenic animal of the invention finds use in identifying anti-cardiopathic compounds.

L'invention porte sur des procédés et des compositions utilisés pour modifier l'expression de préférence cardiaque des animaux transgéniques. Ces compositions contiennent des molécules d'acide nucléique isolées, des cassettes d'expression, ces cellules animales, des animaux transgéniques, et des souris transgéniques. Ces animaux transgéniques présentent une expression de préférence cardiaque inductible d'une séquence nucléotidique d'intérêt. Ces procédés permettent aussi la génération d'animaux transgéniques présentant une expression de préférence cardiaque modifiée de la séquence nucléotidique d'intérêt. Plus particulièrement, l'invention fournit un procédé de modification de la sensibilité d'un animal transgénique à la cardiopathie. Un animal transgénique de l'invention sert à identifier des composés anti-cardiopathiques.

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