A technique to count objects in a scanned image

Details A technique to count objects in a scanned image A technique to count objects in a scanned image

C - Chemistry, Metallurgy

12

M

C12M 1/34 (2006.01) C12Q 1/06 (2006.01) G06M 11/00 (2006.01)

Patent

CA 2138272

The present invention relates to a method and apparatus for counting the number of microorganism colonies present on a disposable microorganism culturing device having a substantially planar substrate. The preferred apparatus (10) of the present in- vention includes an LED bar (20) illuminating the surface of the substrate and a linear array of SELFOC lenses (22) which focus the light reflected off of the substrate onto a linear CCD sensor array (24). The CCD sensor array (24) is connected to a micropro- cessor (32) which analyzes the raster-scanned data using the preferred algorithm. The preferred algorithm used the sigma-shaped neighborhood for image processing. The preferred algorithm passes the sigma region across the image in a raster-scanned order to detect object starts and merges which yield a colony count for a scanned microorganism culturing device.

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