A thermostable ligase mediated dna amplification system for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12N 15/72 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2067991

A THERMOSTABLE LIGASE MEDIATED DNA AMPLIFICATION SYSTEM FOR THE DETECTION OF GENETIC DISEASES ABSTRACT OF THE DISCLOSURE: The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.

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