Aav capsid vehicles for molecular transfer

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 31/70 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C07K 14/015 (2006.01) C12N 7/00 (2006.01) C12N 7/04 (2006.01) C12N 15/35 (2006.01) C12N 15/86 (2006.01) C12N 15/864 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2193802

The invention relates to the production of Adeno-Associated Virus (AAV) capsids which may be used to transfer native or heterologous molecules into appropriate host cells. The capsid proteins can be expressed from a recombinant virus, expression vector, or from a cell line that has stably integrated the AAV capsid genes or coding sequences (as depicted in the Figure). The invention further provides for the production of AAV capsids in vitro from the AAV capsid proteins and the construction of packaged capsids in vitro. The invention further provides for the production of AAV capsids that have been genetically engineered to express heterologous epitopes of clinically important antigens to elicit an immune response.

L'invention se rapporte à la production de capsides de virus adéno-associé (AAV) pouvant être utilisés pour transférer des molécules natives ou hétérologues dans des cellules hôtes appropriées. Les protéines capsidiques peuvent être exprimées à partir d'un virus recombinant, d'un vecteur d'expression ou d'une lignée cellulaire qui a été incorporée de manière stable dans les séquences codantes ou les gènes capsidiques d'AAV (comme illustré dans la figure). L'invention se rapporte en outre à la production in vitro de capsides d'AAV à partir des protéines capsidiques d'AAV, à la construction in vitro de capsides encapsulés, ainsi qu'à des procédés de production de capsides d'AAV qui ont été génétiquement modifiés pour exprimer des épitopes hétérologues d'antigènes présentant un intérêt clinique afin de provoquer une réponse immune.

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